1AQM
ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.400, 138.900, 115.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.000 - 1.850 |
R-factor | 0.157 |
Rwork | 0.157 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PORCINE PANCREAS ALPHA-AMYLASE (QIAN ET AL. J.MOL.BIOL. 231 785-799 (1993)) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.280 * |
Data reduction software | DENZO |
Data scaling software | Agrovata |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.085 * | 0.499 * |
Total number of observations | 153351 * | |
Number of reflections | 42049 | |
<I/σ(I)> | 8.3 | 1.5 |
Completeness [%] | 85.9 | 61.8 |
Redundancy | 3.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 4, 20 * | prtein to mother liquor ration was 2:1 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 60-70 (%) | |
2 | 1 | reservoir | HEPES | 0.1 (M) |