1AQH

ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	71.400, 138.700, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 2.000
R-factor	0.157
Rwork	0.157
R-free	0.20400
Structure solution method	PHASES FROM ALPHA-AMYLASE COMPLEXED WITH A TRIS MOLECULE COMBINED WITH STRUCTURE FACTORS FROM THIS NATIVE DATA SET
RMSD bond length	0.008
RMSD bond angle	1.184 *
Data reduction software	DENZO
Data scaling software	Agrovata
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.142 *	0.142 *
Total number of observations	93724 *
Number of reflections	31485
<I/σ(I)>	5	1.1
Completeness [%]	80.5	31.5
Redundancy	3	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		Aghajari, N., (1996) Protein Sci., 5, 2128. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	60-70 (%)
2	1	reservoir	HEPES	0.1 (M)