1AQF
PYRUVATE KINASE FROM RABBIT MUSCLE WITH MG, K, AND L-PHOSPHOLACTATE
Experimental procedure
| Temperature [K] | 276 |
| Collection date | 1996-02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.400, 112.600, 171.200 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 20.553 * |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.700 * |
| Rmerge | 0.075 * |
| Total number of observations | 736673 * |
| Number of reflections | 76181 * |
| Completeness [%] | 94.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 7.3 (mg/ml) | |
| 2 | 1 | 1 | PEG8000 | 7.7 (%) | |
| 3 | 1 | 1 | 0.21 (M) | ||
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | ATP | 5 (mM) | |
| 6 | 1 | 1 | L-lactate | 4.5 (mM) | |
| 7 | 1 | 1 | HEPES | 50 (mM) | |
| 8 | 1 | 1 | Ap5A | 0.12 (mM) |
