1AQE

CRYSTAL STRUCTURE OF THE Y73E MUTANT OF CYTOCHROME C OF CLASS III (AMBLER) 26 KD

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-03-20
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	72.620, 72.620, 60.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.000 - 2.200
R-factor	0.175
Rwork	0.175
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1czj
RMSD bond length	0.008
RMSD bond angle	26.060 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.096	0.350
Total number of observations	53789 *
Number of reflections	9880
Completeness [%]	95.7	91.1
Redundancy	5.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6	4 *	Czjzek, M., (1992) J. Mol. Biol., 228, 995. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	0.4 (M)
3	1	reservoir	ethanol	13 (%)
4	1	reservoir	Tris-HCl	0.4 (M)