1AQB
RETINOL-BINDING PROTEIN (RBP) FROM PIG PLASMA
Experimental procedure
Source type | ROTATING ANODE |
Source details | MACSCIENCE M18X |
Temperature [K] | 300 |
Detector technology | AREA DETECTOR |
Collection date | 1996-09 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.810, 53.137, 72.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.000 - 1.650 |
R-factor | 0.1844 |
R-free | 0.23720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hbp |
RMSD bond length | 0.030 |
RMSD bond angle | 0.030 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | SHELXL-93 |
Refinement software | SHELXL-93 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 1.720 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.064 | 0.190 |
Total number of observations | 84968 * | |
Number of reflections | 20127 | |
<I/σ(I)> | 8 | 4 |
Completeness [%] | 91.0 | 56 |
Redundancy | 4.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 * | SITTING DROP VAPOR DIFFUSION METHOD, AT A FINAL PROTEIN CONCENTRATION OF 8 MG/ML IN THE PRESENCE OF 8% 2-METHYL-2,4-PENTANEDIOL, 3 MM CADMIUM ACETATE, 0.1 M TRIS-ACETATE, PH=6.8, vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | MPD | 8 (%(v/v)) | |
3 | 1 | reservoir | cadmium acetate | 3 (mM) | |
4 | 1 | reservoir | Tris-acetate | 0.1 (M) |