1AQ8
STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE REDUCED WITH ASCORBATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04-25 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.790, 102.400, 146.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2afn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.600 * |
| Data reduction software | RIGAKU |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.165 |
| Number of reflections | 51866 | 10841 * |
| <I/σ(I)> | 9.4 | 3.1 |
| Completeness [%] | 82.0 | 59 * |
| Redundancy | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 10% PEG4000, 0.1 SODIUM ACETATE PH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 10 (%) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.0-4.8 |
