1AQ3
BACTERIOPHAGE MS2 CAPSID PROTEIN/RNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-28 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 288.000, 288.000, 653.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.204 * |
Rwork | 0.204 |
R-free | 0.25300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | REFINED MODEL OF MS2 PROTEIN CAPSID |
RMSD bond length | 0.017 |
RMSD bond angle | 28.000 * |
Data reduction software | DENZO |
Data scaling software | Agrovata |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.146 | 0.217 |
Number of reflections | 159163 | 4585 * |
<I/σ(I)> | 3.4 | 2.8 |
Completeness [%] | 63.2 * | 25.1 * |
Redundancy | 1.8 | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 | 37 * | pH 7.40 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MS2 | 1.0 (%(w/v)) | |
2 | 1 | drop | sodium phosphate | 0.2 (M) | |
3 | 1 | drop | PEG6000 | 1.5 (%(w/v)) | |
4 | 1 | drop | 0.02 (%(w/v)) | ||
5 | 1 | reservoir | sodium phosphate | 0.4 (M) |