1AQ0
BARLEY 1,3-1,4-BETA-GLUCANASE IN MONOCLINIC SPACE GROUP
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 294.2 |
Detector technology | IMAGE PLATE |
Collection date | 1992-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.580, 82.990, 77.560 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 13.600 - 2.000 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ghr |
RMSD bond length | 0.007 |
RMSD bond angle | 24.060 * |
Data reduction software | MOSFLM |
Data scaling software | Agrovata |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.650 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 * | |
Number of reflections | 40727 | |
Completeness [%] | 99.2 | 96.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | Keitel, T., (1993) J.Mol.Biol., 232, 1003. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | 60 (mM) | ||
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | PEG8000 | 10 (%(w/w)) | |
5 | 1 | reservoir | 100 (mM) | ||
6 | 1 | reservoir | PEG8000 | 20 (%(w/w)) |