1APL
CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.780, 39.930, 68.790 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.600 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.650 * |
| Rmerge | 0.075 * |
| Total number of observations | 18265 * |
| Number of reflections | 7907 * |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | referred to J.Mol.Biol. 217.11-13 1991 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | ||
| 2 | 1 | drop | DNA | ||
| 3 | 1 | reservoir | PEG400 | 10 (%) | |
| 4 | 1 | reservoir | 75 (mM) |
