1APH

CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11

Experimental procedure

Spacegroup name	I 21 3
Unit cell lengths	78.900, 78.900, 78.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.000
R-factor	0.02 *
RMSD bond length	0.017
RMSD bond angle	0.051 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.095 *
Total number of observations	21745 *
Number of reflections	5431 *
Completeness [%]	100.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	9.2 *		referred to 'Dodson, E.J.', (1978) J.Mol.Biol., 125, 387-396 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Na3EDTA	0.01 (M)
3	1	2		0.2 (M)