1APB

A PRO TO GLY MUTATION IN THE HINGE OF THE ARABINOSE-BINDING PROTEIN ENHANCES BINDING AND ALTERS SPECIFICITY: SUGAR-BINDING AND CRYSTALLOGRAPHIC STUDIES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	55.460, 71.820, 77.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.760
R-factor	0.192
RMSD bond length	0.004
RMSD bond angle	0.011
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.760 *
Total number of observations	68318 *
Number of reflections	25278 *
Completeness [%]	79.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG	10 (%)
3	1	drop	citrate	10 (mM)
4	1	drop		1 (mM)
5	1	drop	sodium azide	0.02 (%)
6	1	drop	D-fucose	10 (mM)
7	1	reservoir	PEG	20 (%)