1AP9
X-RAY STRUCTURE OF BACTERIORHODOPSIN FROM MICROCRYSTALS GROWN IN LIPIDIC CUBIC PHASES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.760, 61.760, 104.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.350 |
| R-factor | 0.2582 |
| Rwork | 0.258 |
| R-free | 0.31790 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 20.823 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.560 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 * | |
| Number of reflections | 8045 | |
| <I/σ(I)> | 4.7 | 2.4 |
| Completeness [%] | 91.2 | 90.4 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
| 2 | 1 | 1 | protein | 3.5 (mg/ml) | |
| 3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
| 4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |
