1AON
CRYSTAL STRUCTURE OF THE ASYMMETRIC CHAPERONIN COMPLEX GROEL/GROES/(ADP)7
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 255.260, 265.250, 184.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oel |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 * | 0.530 * |
| Number of reflections | 242684 | 28317 * |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 96.7 | 91.2 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | 18 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3000 | 12 (%) | |
| 2 | 1 | reservoir | sodium glutamate | 0.25 (M) | |
| 3 | 1 | reservoir | cacodylate | 100 (mM) | |
| 4 | 1 | drop | PEG3000 | 6 (%) | |
| 5 | 1 | drop | sodium glutamate | 0.125 (M) | |
| 6 | 1 | drop | cacodylate | 50 (mM) | |
| 7 | 1 | drop | protein | 7.5 (mg/ml) |
