1AOM

SUBSTRATE AND PRODUCT BOUND TO CYTOCHROME CD1 NITRITE REDUCTASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-04-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	106.700, 60.600, 100.100
Unit cell angles	90.00, 112.30, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.181
Rwork	0.181
R-free	0.20000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.046 *
Total number of observations	299460 *
Number of reflections	98949
Completeness [%]	90.2	77.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	15 *	Fulop, V., (1993) J. Mol. Biol., 232, 1211. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	1.1-1.15 (M)
3	1	drop	potassium phosphate	25 (mM)
4	1	reservoir	ammonium sulfate	2.2-2.3 (M)
5	1	reservoir	potassium phosphate	50 (mM)