1AO6
CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-05-10 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 59.680, 96.980, 59.720 |
Unit cell angles | 91.07, 103.50, 75.08 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A PARTIAL MODEL FROM MIR EXPERIMENT WITH THE KNOWN TETRAGONAL CRYSTAL FORM |
RMSD bond length | 0.014 |
RMSD bond angle | 21.000 * |
Data reduction software | PROCESS |
Data scaling software | PROCESS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.620 | 2.500 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.097 | 0.272 |
Number of reflections | 40737 | |
<I/σ(I)> | 9.35 | 2.41 |
Completeness [%] | 81.4 | 46.9 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 293 * | PROTEIN WAS CRYSTALLIZED FROM 22-25% PEG 3350, 50 MM POTASSIUM PHOSPHATE, PH 7.0-7.5. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 150-255 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | drop | PEG400 | 30-38 (%(v/v)) | |
4 | 1 | drop | sodium azide | 5 (mM) |