1AO6

CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-05-10
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	59.680, 96.980, 59.720
Unit cell angles	91.07, 103.50, 75.08

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.218
Rwork	0.218
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A PARTIAL MODEL FROM MIR EXPERIMENT WITH THE KNOWN TETRAGONAL CRYSTAL FORM
RMSD bond length	0.014
RMSD bond angle	21.000 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.620	2.500
High resolution limit [Å]	2.380	2.380
Rmerge	0.097	0.272
Number of reflections	40737
<I/σ(I)>	9.35	2.41
Completeness [%]	81.4	46.9
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293 *	PROTEIN WAS CRYSTALLIZED FROM 22-25% PEG 3350, 50 MM POTASSIUM PHOSPHATE, PH 7.0-7.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	150-255 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	PEG400	30-38 (%(v/v))
4	1	drop	sodium azide	5 (mM)