1AO5
MOUSE GLANDULAR KALLIKREIN-13 (PRORENIN CONVERTING ENZYME)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.750, 73.750, 192.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.330 - 2.600 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kai |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.808 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.330 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.065 | 0.345 |
| Number of reflections | 17034 | |
| <I/σ(I)> | 9.6 | 1.7 |
| Completeness [%] | 95.3 | 86.8 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | MGK-13 (57 MG/ML) WAS CRYSTALLIZED USING 10-15% PEG8000, 0.2M LISO4, 0.1M SODIUM CACODYLATE, PH 6.5 BY HANGING DROP VAPOR DIFFUSION METHOD., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | mGK-13 | 57 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10-15 (%) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | sodium cacodylate | 100 (mM) |
