1ANI
ALKALINE PHOSPHATASE (D153H, K328H)
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1993-10-28 |
Detector | SAN DIEGO MULTIWIRE |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 194.600, 167.300, 76.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.23300 * |
RMSD bond length | 0.019 |
RMSD bond angle | 24.200 * |
Data reduction software | SDMS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.072 |
Total number of observations | 118201 * |
Number of reflections | 39775 |
Completeness [%] | 91.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9.5 * | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 30 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
3 | 1 | drop | Tris-HCl | 100 (mM) | |
4 | 1 | drop | 10 (mM) | ||
5 | 1 | drop | 0.01 (mM) | ||
6 | 1 | reservoir | ammonium sulfate | 36-39 (%sat) |