Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1989-01 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.880, 68.440, 57.940 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.670 |
| R-factor | 0.182 * |
| Rwork | 0.182 |
| Structure solution method | ISOMORPHOUS WITH 2MBP |
| Starting model (for MR) | 2MBP |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.055 |
| Data reduction software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Data scaling software | SDMS (DETECTOR SYSTEM (NIELSEN)) |
| Phasing software | PROTEIN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.700 |
| Rmerge | 0.065 | 0.180 |
| Number of reflections | 39297 | |
| <I/σ(I)> | 13.88 | 2.5 |
| Completeness [%] | 90.0 | 68 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 | 4 * | PROTEIN WAS CRYSTALLIZED FROM 18% PEG 8000, 10 MM CITRATE, PH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MBP | 3.5 (mg/ml) | |
| 2 | 1 | drop | maltodextrin | 1 (mM) | |
| 3 | 1 | drop | PEG6000 | 16 (%(w/v)) | |
| 4 | 1 | reservoir | PEG6000 | 22 (%(w/v)) |
