1AND

ANIONIC TRYPSIN MUTANT WITH ARG 96 REPLACED BY HIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	273
Detector technology	AREA DETECTOR
Collection date	1992
Detector	SIEMENS
Spacegroup name	I 2 3
Unit cell lengths	124.380, 124.380, 124.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.161
Rwork	0.161
RMSD bond length	0.013
RMSD bond angle	2.900
Data reduction software	BUDDHA
Data scaling software	BUDDHA
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300
Rmerge	0.070 *
Total number of observations	26500 *
Number of reflections	13200
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		22 (%(w/v))
2	1	reservoir	Tris-HCl	20 (mM)