1ANC

ANIONIC TRYPSIN MUTANT WITH SER 214 REPLACED BY LYS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	273
Detector technology	AREA DETECTOR
Collection date	1990
Detector	SIEMENS
Spacegroup name	I 2 3
Unit cell lengths	124.380, 124.380, 124.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.154 *
Rwork	0.154
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.012
RMSD bond angle	3.100 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.091 *
Total number of observations	52900 *
Number of reflections	17000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8.0