1AN8
CRYSTAL STRUCTURE OF THE STREPTOCOCCAL SUPERANTIGEN SPE-C
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY |
Synchrotron site | Photon Factory |
Temperature [K] | 293 |
Detector technology | FILM |
Collection date | 1996-04 |
Detector | FILM |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.410, 75.410, 168.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.174 |
Rwork | 0.171 |
R-free | 0.21500 * |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 22.127 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | CCP4 |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.570 |
High resolution limit [Å] | 2.400 | 2.420 |
Rmerge | 0.103 * | 0.423 * |
Total number of observations | 71127 * | |
Number of reflections | 17866 | |
<I/σ(I)> | 5.9 | 2.4 |
Completeness [%] | 91.6 | 87.4 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 | PROTEIN WAS CRYSTALLIZED FROM 2.7 M AMMONIUM SULFATE IN 0.1 M TRIS/HCL BUFFER AT PH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.5 (mg/ml) | |
2 | 1 | drop | phosphate | 0.1 (M) | |
3 | 1 | reservoir | ammonium sulfate | 2.7 (M) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |