1AN7

RIBOSOMAL PROTEIN S8 FROM THERMUS THERMOPHILUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-02-10
Detector	MAR scanner 300 mm plate
Spacegroup name	P 41 21 2
Unit cell lengths	67.860, 67.860, 174.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.162
Rwork	0.162
R-free	0.27500
Structure solution method	MIR
RMSD bond length	0.016
RMSD bond angle	25.291 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.051	0.019
Number of reflections	8932
Completeness [%]	94.5	93.6
Redundancy	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	24 *	Tishchenko, S.V., (1997) Proteins: Struct.,Funct., Genet., 27, 309. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	potassium phosphate	40 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	ammonium sulfate	40 (%sat)
5	1	reservoir	MPD	7 (%(v/v))