1AN4
STRUCTURE AND FUNCTION OF THE B/HLH/Z DOMAIN OF USF
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 253 |
Detector technology | IMAGE PLATE |
Collection date | 1993-06-01 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 136.600, 54.700, 44.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.900 |
Rwork | 0.236 |
RMSD bond length | 0.019 |
RMSD bond angle | 3.050 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.076 | |
Total number of observations | 32049 * | |
Number of reflections | 6038 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 76.4 * | 49.3 |
Redundancy | 2.6 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | GLYCEROL | ||
11 | 1 | 2 | KCL | ||
12 | 1 | 2 | MGCL2 | ||
13 | 1 | 2 | CD ACETATE | ||
14 | 1 | 2 | NA ACETATE | ||
2 | 1 | 1 | PEG 400 | ||
3 | 1 | 1 | GLYCEROL | ||
4 | 1 | 1 | KCL | ||
5 | 1 | 1 | MGCL2 | ||
6 | 1 | 1 | CD ACETATE | ||
7 | 1 | 1 | NA ACETATE | ||
8 | 1 | 2 | WATER | ||
9 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1.4 (mM) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | HEPES | 10 (mM) | |
4 | 1 | reservoir | PEG400 | 15 (%) | |
5 | 1 | reservoir | glycerol | 15 (%) | |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | 2.8 (mM) | ||
8 | 1 | reservoir | Cd(II) acetate | 1.4 (mM) | |
9 | 1 | reservoir | sodium acetate | 100 (mM) |