1AMS

X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAMINE 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN THREE FORMS

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	155.100, 86.600, 79.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.243
Rwork	0.243
RMSD bond length	0.007
RMSD bond angle	1.467
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.062 *
Total number of observations	39393 *
Number of reflections	15402 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	40 (%sat)
2	1	drop	protein	40-60 (mg/ml)
3	1	drop	potassium phosphate	10 (mM)
4	1	drop	PMP	0.01 (mM)
5	1	drop		0.3 (mM)
6	1	reservoir	ammonium sulfate	40 (%sat)