1AMP

CRYSTAL STRUCTURE OF AEROMONAS PROTEOLYTICA AMINOPEPTIDASE: A PROTOTYPICAL MEMBER OF THE CO-CATALYTIC ZINC ENZYME FAMILY

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	111.000, 111.000, 92.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.161
Rwork	0.161
RMSD bond length	0.014
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.051 *
Total number of observations	367007 *
Number of reflections	31134 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *	Schalk, C., (1992) Arch.Biochem.Biophys., 294, 91. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	drop		10 (mM)
4	1	drop		0.4 (M)
5	1	reservoir	Tris	100 (mM)
6	1	reservoir		100 (mM)
7	1	reservoir		4.5 (M)