1AMF
CRYSTAL STRUCTURE OF MODA, A MOLYBDATE TRANSPORT PROTEIN, COMPLEXED WITH MOLYBDATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.570, 82.570, 81.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.20300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLOMON |
| Refinement software | X-PLOR (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 * | 0.244 * |
| Total number of observations | 112116 * | |
| Number of reflections | 30575 | |
| <I/σ(I)> | 21 | 1.9 |
| Completeness [%] | 93.2 | 60.8 |
| Redundancy | 3.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.7 | PROTEIN WAS CRYSTALLIZED FROM 27.5% PEG 8000, 0.1M SODIUM ACETATE, PH 4.7, AND 2MM SODIUM MOLYBDATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | 2 (mM) | or Na2WO4 | |
| 4 | 1 | reservoir | PEG8000 | 27.5 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
