1AME

CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN AT 4 C

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-02
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	32.690, 38.840, 47.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.650
R-factor	0.199
Rwork	0.199
R-free	0.28200
Structure solution method	ISOMORPHOUS (ROOM TEMPERATURE STARTING MODEL)
Starting model (for MR)	1msi
RMSD bond length	0.019
RMSD bond angle	26.970 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.061 *	0.397
Total number of observations	27105 *
Number of reflections	6600
<I/σ(I)>	15.13	2.29
Completeness [%]	86.6	85.8
Redundancy	4.07	4.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	277	drop was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	53-55 (%)
3	1	reservoir	sodium acetate	0.1 (M)