1AM5
THE CRYSTAL STRUCTURE AND PROPOSED AMINO ACID SEQUENCE OF A PEPSIN FROM ATLANTIC COD (GADUS MORHUA)
Experimental procedure
| Source details | BRUKER NONIUS |
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-10 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.980, 75.400, 108.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.160 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5pep |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.071 |
| Data reduction software | CCP4 |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.066 * | 0.221 * |
| Total number of observations | 37108 * | |
| Number of reflections | 13687 | 948 * |
| <I/σ(I)> | 10.8 | 3.5 |
| Completeness [%] | 83.7 | 41.4 |
| Redundancy | 2.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 | 277 * | 7.5 % 2-PROPANOL, 100 MM SODIUM ACETATE PH 5.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 2-propanol | 3.75 (%) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 7.5 (%) | |
| 5 | 1 | reservoir | 2-propanol | 100 (mM) |
