1ALL

ALLOPHYCOCYANIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	268
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	101.900, 101.900, 130.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.196
Rwork	0.196
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	1.409
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	999.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.070
Total number of observations	177454 *
Number of reflections	17566
Completeness [%]	95.3	68.6
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	APC	10 (mg/ml)
2	1	drop	potassium phosphate	5 (mM)
3	1	reservoir		0.1 (M)
4	1	reservoir	Tris-HCl	50 (mM)
5	1	reservoir	PEG4000	15 (%(w/v))