1ALH
KINETICS AND CRYSTAL STRUCTURE OF A MUTANT E. COLI ALKALINE PHOSPHATASE (ASP-369-->ASN): A MECHANISM INVOLVING ONE ZINC PER ACTIVE SITE
Experimental procedure
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 194.392, 167.251, 76.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond angle | 2.490 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 42809 |
| Completeness [%] | 98.4 |
| Redundancy | 7.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 30 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulphate | 20 (%sat) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | 0.01 (mM) |
