1ALB

CRYSTAL STRUCTURE OF RECOMBINANT MURINE ADIPOCYTE LIPID-BINDING PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	34.400, 54.800, 76.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.183
Rwork	0.183
RMSD bond length	0.017
RMSD bond angle	3.460
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.500 *
Total number of observations	5115 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	19 *	used as seeds *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ALBP	2.5 (mg/ml)
2	1	drop	Tris	0.05 (M)
3	1	drop	ammonium sulfate	60 (%)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	drop	sodium azide	0.05 (%)
7	1	reservoir	ammonium sulfate	80 (%sat)
8	1	reservoir	Tris	0.05 (M)