1AKY
HIGH-RESOLUTION STRUCTURES OF ADENYLATE KINASE FROM YEAST LIGATED WITH INHIBITOR AP5A, SHOWING THE PATHWAY OF PHOSPHORYL TRANSFER
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1990 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.300, 40.500, 45.700 |
| Unit cell angles | 110.80, 109.00, 63.30 |
Refinement procedure
| Resolution | 10.000 - 1.630 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9999.000 * |
| High resolution limit [Å] | 1.630 |
| Rmerge | 0.076 |
| Number of reflections | 27634 |
| Completeness [%] | 98.0 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Ap5A | 1.1 (mM) | |
| 3 | 1 | drop | PEG8000 | 26 (%) | |
| 4 | 1 | drop | Im | 60 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 30 (%) | |
| 6 | 1 | reservoir | Im | 60 (mM) |
