1AKR
G61A OXIDIZED FLAVODOXIN MUTANT
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 56.493, 87.990, 35.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 1.580 |
R-factor | 0.184 * |
Rwork | 0.184 |
R-free | 0.20600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.030 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.580 |
High resolution limit [Å] | 1.580 | 1.560 |
Rmerge | 0.036 * | 0.341 * |
Total number of observations | 126829 * | |
Number of reflections | 24403 | |
<I/σ(I)> | 19 | 1.6 |
Completeness [%] | 98.9 | 87.6 * |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 18 * | 60-70% AMMONIUM SULFATE, 100MM TRIS-HCL, pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | acetone | 1-2 (%(v/v)) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | ammonium sulfate | 60-70 (%) |