1AKQ
D95A OXIDIZED FLAVODOXIN MUTANT FROM D. VULGARIS
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 52.805, 52.805, 139.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.196 * |
Rwork | 0.190 |
R-free | 0.23630 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.850 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.520 | 0.298 * |
Total number of observations | 70384 * | |
Number of reflections | 17939 | |
<I/σ(I)> | 16.68 | 1.2 |
Completeness [%] | 98.0 | 97.6 * |
Redundancy | 2.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 18 * | PROTEIN WAS CRYSTALLIZED FROM 60-70% A.S., 100MM TRIS-HCL, PH=7.0. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12-15 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 30-35 (%sat) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | or 25mM sodium phosphate containing 1mM EDTA |
4 | 1 | reservoir | ammonium sulfate | 60-70 (%sat) |