1AKO
EXONUCLEASE III FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 292 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-05 |
| Detector | XENTRONICS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.800, 107.800, 42.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.20200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.190 * |
| Data reduction software | XENGEN (V. 1.3) |
| Data scaling software | XENGEN (V. 1.3) |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 * | |
| Total number of observations | 199757 * | |
| Number of reflections | 28817 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 92.0 | 62 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 13% PEG 4000, 1.0 M IMIDAZOLE/ MALATE, PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 13 (%) | |
| 2 | 1 | 1 | imidazole/malate | 1.0 (M) |
