1AKD

CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	277
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1996-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	64.300, 66.200, 106.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 1.800
R-factor	0.209
Rwork	0.209
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNPUBLISHED COORDINATES FROM TETRAGONAL FORM OF P450CAM
RMSD bond length	0.009
RMSD bond angle	34.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.046	0.284
Total number of observations	143928 *
Number of reflections	41095
Completeness [%]	92.0	69.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4	4 *	PROTEIN WAS CRYSTALLIZED BY DIALYSIS FROM 10% PEG 8000, 50 MM TRISHCL PH 7.4, 250 MM KCL, 100 MM DTE, IN THE PRESENCE OF EXCESS (1S)-CAMPHOR., dialysis

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	reservoir	Tris-HCl	50 (mM)
3	1	reservoir		250 (mM)
4	1	reservoir	DTE	100 (mM)
5	1	reservoir	PEG8000	10 (%)