1AKB

STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING ITS PYRIDOXAL-5'-PHOSPHATE-BINDING LYSINE RESIDUE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	70.100, 91.800, 129.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.178
RMSD bond length	0.010
RMSD bond angle	0.036
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.082 *
Total number of observations	72346 *
Number of reflections	18825 *
Completeness [%]	96.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG	9-14 (%)
2	1	drop	sodium phosphate	20 (mM)
3	1	drop		0.9 (M)
4	1	drop	PPxy-Asp	20 (mM)
5	1	drop	apoenzyme	10 (mg/ml)
6	1	reservoir	PEG	18-28 (%)
7	1	reservoir	sodium phosphate	20 (mM)