1AK0
P1 NUCLEASE IN COMPLEX WITH A SUBSTRATE ANALOG
Experimental procedure
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.980, 74.040, 102.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.950 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 * | 0.247 * |
| Total number of observations | 119199 * | |
| Number of reflections | 27854 | |
| Completeness [%] | 93.2 | 89.9 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 | PEG 6000 12 - 20% 25 MM NA ACETATE PH 5.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.8 (mM) | |
| 2 | 1 | drop | DNA | ||
| 3 | 1 | drop | reservoir | ||
| 4 | 1 | reservoir | sodium acetate | 25 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 12-20 (%(w/v)) | |
| 6 | 1 | reservoir | zinc chloride | 2 (mM) |
