1AJO
CIRCULARLY PERMUTED (1-3,1-4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE CPA16M-127
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 294 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 44.950, 46.550, 63.990 |
Unit cell angles | 92.60, 109.28, 116.68 |
Refinement procedure
Resolution | 9.990 - 2.070 |
Rwork | 0.176 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayh |
RMSD bond length | 0.013 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.620 | 2.150 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.109 | 0.196 |
Total number of observations | 43819 * | |
Number of reflections | 24275 | |
<I/σ(I)> | 2.94 | 3.3 |
Completeness [%] | 93.5 | 90 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | reservoir | imidazol | 0.1 (M) | |
5 | 1 | reservoir | 2 (mM) | ||
6 | 1 | reservoir | PEG8000 | 10 (%) | |
7 | 1 | reservoir | ethylene glycol | 8 (%) |