1AJJ

LDL RECEPTOR LIGAND-BINDING MODULE 5, CALCIUM-COORDINATING

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1996-11
Detector	RIGAKU
Spacegroup name	H 3
Unit cell lengths	53.450, 53.450, 26.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.209
Rwork	0.209
R-free	0.24000
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	2.498
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.054 *
Number of reflections	2959
<I/σ(I)>	20	7
Completeness [%]	94.3	87.7
Redundancy	4.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	15 *	PROTEIN WAS CRYSTALLIZED FROM 2.1 M AMMONIUM SULFATE, PH 5.0, 25% SUCROSE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		5 (mM)
3	1	reservoir	ammonium sulfate	2.1 (M)	pH5.0
4	1	reservoir	sucrose	25 (%)