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THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01-06 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 195.020, 166.930, 76.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 24.900 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.077 |
| Number of reflections | 40175 |
| Completeness [%] | 77.0 |
| Redundancy | 1.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | reservoir | zinc chloride | 0.01 (mM) | |
| 3 | 1 | reservoir | magnesium sulfate | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 25 (%sat) |
