1AJ5

CALPAIN DOMAIN VI APO

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-06-24
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	67.500, 73.000, 156.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.22
Rwork	0.220
R-free	0.30600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	20.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.040	0.190
Total number of observations	80668 *
Number of reflections	17172
<I/σ(I)>	23
Completeness [%]	97.7	94.2 *
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8		38%SATURATED AMMONIUM SULFATE AND 25MM PIPES BUFFER, PH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate