1AIV

APO OVOTRANSFERRIN

Experimental procedure

Temperature [K]	123
Detector technology	AREA DETECTOR
Collection date	1996-12-20
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	92.260, 92.260, 178.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.231
Rwork	0.231
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ovt
RMSD bond length	0.010
RMSD bond angle	24.413 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.000
Rmerge	0.069 *
Total number of observations	31370 *
Number of reflections	12920
<I/σ(I)>	6.5
Completeness [%]	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	4 *	pH 5.9

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	42.5 (mg/ml)
2	1	drop	acetate	0.02 (M)
3	1	drop	PEG6000	2-3 (%)
4	1	reservoir	PEG6000	4-6 (%)
5	1	reservoir	acetate	0.02 (M)