1AIR
PECTATE LYASE C FROM ERWINIA CHRYSANTHEMI (EC16) TO A RESOLUTION OF 2.2 ANGSTROMS WITH 128 WATERS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 291 |
Detector technology | AREA DETECTOR |
Collection date | 1990-02 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.000, 83.000, 95.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.22300 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.199 * |
Data reduction software | SDMS (SOFTWARE) |
Data scaling software | SDMS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.175 |
Number of reflections | 25685 |
Completeness [%] | 95.1 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 * | 4 * | Kim, C-Y., (1989) J. Mol. Biol., 208, 365. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | HEPES | 25 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | polygalacturonic acid | 20 (%sat) | |
5 | 1 | drop | PEG3350 | 10 (%(w/w)) | |
6 | 1 | reservoir | PEG3350 | 20ml |