1AIP
EF-TU EF-TS COMPLEX FROM THERMUS THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.300, 127.500, 123.700 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.28900 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.150 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.020 |
| Rmerge | 0.080 * | 0.372 * |
| Number of reflections | 69409 | |
| <I/σ(I)> | 15 | 3.96 |
| Completeness [%] | 92.0 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 60 MM CACODYLATE, PH 6.5 70 MM AMMONIUM SULFATE 8% PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 8 (%) | |
| 2 | 1 | drop | Tris-Cl | 5 (mM) | |
| 3 | 1 | drop | 5 (mM ) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | cacodylate | 60 (mM) | |
| 6 | 1 | drop | ammonium sulfate | 70 (mM) | |
| 7 | 1 | drop | PEG8000 | 8 (%) | |
| 8 | 1 | reservoir | cacodylate | 60 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 70 (mM) |
