1AIH
CATALYTIC DOMAIN OF BACTERIOPHAGE HP1 INTEGRASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 1996-12-12 |
Detector | PRINCETON 2K |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.400, 129.300, 234.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 22.480 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.780 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.087 | 0.180 |
Total number of observations | 52126 * | |
Number of reflections | 34819 | |
<I/σ(I)> | 11.4 | 5 |
Completeness [%] | 97.0 | 89.7 |
Redundancy | 6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | VAPOR DIFFUSION, 15% PEG 8000, 45MM NA CAODYLATE, 34MM AMMONIUM SULFATE, 10MM TRIS-HCL, 4.5% GLYCEROL, 0.1 M MGCL., pH 7.5, vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 15-17 (%) | |
3 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
5 | 1 | drop | ammonium sulfate |