1AIC

STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING THE PYRIDOXAL-5'-PHOSPHATE BINDING LYSINE RESIDUE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	87.200, 79.900, 89.600
Unit cell angles	90.00, 119.10, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.191
Rwork	0.191
RMSD bond length	0.013
RMSD bond angle	3.120
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.047 *
Number of reflections	72346 *
Completeness [%]	93.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15.1 (mg/mL)
2	1	drop	maleate	100 (mM)
3	1	drop	sodium chloride	0.45-0.55 (M)
4	1	drop	PEG	16 (%(w/v))
5	1	drop	sodium phosphate	20 (mM)
6	1	reservoir	PEG	32 (%(w/v))
7	1	reservoir	sodium chloride	0.9-1.1 (M)
8	1	reservoir	sodium phosphate	20 (mM)