1AHT
CRYSTAL STRUCTURE OF HUMAN ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND P-AMIDINOPHENYLPYRUVATE AT 1.6 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.240, 72.390, 73.060 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | ? - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.600 * |
| Rmerge | 0.046 * |
| Total number of observations | 84716 * |
| Number of reflections | 36488 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | thrombin-hirugen complex | 3.7 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | |
| 3 | 1 | drop | 0.375 (M) | ||
| 4 | 1 | drop | 0.5 (mM) | ||
| 5 | 1 | reservoir | phosphate | 0.1 (M) | |
| 6 | 1 | reservoir | 1 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |
