1AHS
CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7
Experimental procedure
Source type | ROTATING ANODE |
Detector technology | IMAGE PLATE |
Collection date | 1993-06-15 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 157.200, 157.200, 57.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.213 |
Rwork | 0.213 |
Structure solution method | MIR/MR |
Starting model (for MR) | 1bvp |
RMSD bond length | 0.017 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.089 |
Number of reflections | 29456 |
Completeness [%] | 91.0 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | urea | 2.8 (M) | |
3 | 1 | drop | protein | 6-8 (mg/ml) | |
4 | 1 | reservoir | ammonium sulfate | 45-48 (%sat) | |
5 | 1 | reservoir | Tris-HCl | 10 (mM) |