1AHR
CALMODULIN MUTANT WITH A TWO RESIDUE DELETION IN THE CENTRAL HELIX
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 300 |
Detector technology | AREA DETECTOR |
Collection date | 1988-12 |
Detector | ADSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.090, 55.510, 100.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.211 |
Rwork | 0.211 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cln |
RMSD bond length | 0.015 |
RMSD bond angle | 1.800 |
Data reduction software | UCSD |
Data scaling software | ROCKS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 |
Rmerge | 0.050 * |
Total number of observations | 27799 * |
Number of reflections | 14407 |
<I/σ(I)> | 8.68 |
Completeness [%] | 67.3 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.25 | 28-33% MPD, 15% ETHANOL, 5MM CACL, 20MM NA ACETATE, PH 4.25 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11.7 (mg/ml) | |
2 | 1 | drop | MPD | 28-33 (%) | |
3 | 1 | drop | ethanol | 15 (%) | |
4 | 1 | drop | calcium chloride | 5 (mM) | |
5 | 1 | drop | sodium acetate | 20 (mM) |