1AHR

CALMODULIN MUTANT WITH A TWO RESIDUE DELETION IN THE CENTRAL HELIX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	1988-12
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	27.090, 55.510, 100.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.211
Rwork	0.211
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cln
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	UCSD
Data scaling software	ROCKS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800
Rmerge	0.050 *
Total number of observations	27799 *
Number of reflections	14407
<I/σ(I)>	8.68
Completeness [%]	67.3
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.25		28-33% MPD, 15% ETHANOL, 5MM CACL, 20MM NA ACETATE, PH 4.25

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11.7 (mg/ml)
2	1	drop	MPD	28-33 (%)
3	1	drop	ethanol	15 (%)
4	1	drop	calcium chloride	5 (mM)
5	1	drop	sodium acetate	20 (mM)